Effect of the continuous positive airway pressure on refractory hypertension in patients with obstructive sleep apnea hypopnea syndrome / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the effect of continuous positive airway pressure(CPAP) on blood pressure in patients with obstructive sleep apnea hypopnea syndrome(OSAHS) and refractory hypertension.METHODS Fifty five OSAHS patients with refractory hypertension were treated with CPAP.Ambulatory blood pressure monitoring was performed to the patients before and 6 months after the treatment.RESULTS After treatment,the blood pressure of all the patients decreased at different degrees.The mean of 24 h systolic blood pressure(SBP) and 24 h diastolic blood pressure(DBP) decreased from (136.4±2.8)mmHg and (85.6± 1.7)mmHg to (126.9± 1.0)mmHg and (76.8± 1.3)mmHg,the differences were statistically significant(P＜0.05).The mean decrease of 24 h SBP and DBP were (9.6 ± 3.0)mmHg and (8.7± 2.0)mmHg.The decrease value of 24 h SBP and DBP were concerned with apnea and hypopnea index.The numbers of antihypertensive drugs decreased in 31 patients.CONCLUSION After CPAP treatment,the blood pressure of the patients with OSAHS and refractory hypertension dropped significantly,and the varieties of antihypertensive drugs taken in these patients were reduced significantly.

Effect of the obstructive sleep apnea hypopnea syndrome treatment on blood pressure in patients with resistant hypertension / 中华耳鼻咽喉头颈外科杂志

Objective@#To investigate the effect of the treatments for obstructive sleep apnea hypopnea syndrome (OSAHS) on the resistant hypertension (RH) of patients.@*Methods@#Eighty patients with OSAHS and RH (blood pressure could not be controlled under 140/90 mmHg (1 mmHg=0.133 kPa) even with more than three kinds of antihypertensive drugs including diuretics) received surgery or continuous positive airway pressure (CPAP) treatment. The results of polysomnography monitoring, ambulatory blood pressure monitoring, and the dosage of antihypertensive medication were recorded before and six months after the treatment.@*Results@#Apnea hypopnea index (AHI) decreased from (32.9±10.8) before treatment to (9.4±6.5) after treatment, while the lowest oxygen saturation (SaO2) increased from (0.682±0.062) to (0.884±0.056), with significant differences (t value was 18.863 and 26.614, respectively; both P<0.001). Twenty-four hours systolic blood pressure (SBP)/diastolic blood pressure (DBP) decreased respectively from ((150.5±9.8)/(97.8±7.3)) mmHg to ((140.7±6.8)/(88.6±6.3)) mmHg, daytime SBP/DBP decreased from ((154.3±8.9)/(100.6±7.4)) mmHg to ((144.8±5.8)/(91.3±5.5)) mmHg, and nighttime SBP/DBP decreased from ((145.5±8.8)/(93.8±6.4)) mmHg to ((135.8±5.7)/(84.6±5.9)) mmHg, with significant differences (t value was 7.832, 6.903, 7.005, 6.848, 8.025, 7.554, respectively; all P<0.001). The reduction of nighttime SBP /DBP was ((11.5±2.2)/(10.2±3.1)) mmHg, and the reduction of daytime SBP/DBP was ((9.0±2.8)/(7.9±3.5)) mmHg. The reduction of nighttime SBP/DBP was more obvious than daytime SBP/DBP, with significant differences (t value was 9.732 and 6.936, respectively; both P<0.001). Before treatment, nighttime blood pressure decrease rate below 10% was showed in 75 percent of patients, and after treatment, this rate only in 37.5 percent of patients (χ2=22.857, P<0.01). The numbers of required antihypertensive drugs decreased in 45 (56.3%) cases, the average numbers of antihypertensive drugs decreased from (3.2±0.4) before treatment to (2.6±0.5) after treatment, with a significant difference (t=9.276, P<0.01).@*Conclusions@#After treatment of OSAHS, the blood pressure of the patients with OSAHS and RH dropped significantly, the circadian rhythm of blood pressure condition was better, the varieties of antihypertensive drugs taken in these patients were reduced significantly.

One case of parotid eosinophilic lymphogranuloma / 临床耳鼻咽喉头颈外科杂志

The clinical manifestation was painless mass in the parotid gland. Physical examination showed regional swelling in parotid area. Bultrasound examination demonstrated the mass was an hypoechoic nodules of bilateral parotid gland, the border was vague. Absolute value and ratio of peripheral eosinophils were both significantly increased. Pathological examination: parotid eosinophilic lymphogranuloma.

Investigation of chronic rhinosinusitis on junior middle school students in Zhengzhou area in 2009 / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate the incidence of chronic rhinosinusitis and its influence on life quality for junior middle school students in Zhengzhou Municipal.</p><p><b>METHODS</b>Two thousand and twenty junior middle school students in Zhengzhou Municipal were randomly selected as the object of investigation by designed survey and visual analogue scale (VAS) questionnaire of chronic rhinosinusitis the sino-nasal outcome test-20 (SNOT-20) and nasal examination. Effect on the quality of life was investigated based on the degree of troubles caused by symptoms.</p><p><b>RESULTS</b>The incidence of chronic rhinosinusitis in junior middle school students in Zhengzhou Municipal was 6.73% (136/2 020), in which, 22.79% (31/136) of these students' quality of life was affected (VAS>5).</p><p><b>CONCLUSIONS</b>Chronic rhinosinusitis is common in junior middle school students, and the life quality is affected. So we should pay attention to this diseases and take the positive and effective intervention measures.</p>

Optimization of plant des-pGlu1-Brazzein gene according to yeasty biased codons and its expression in Pichia pastoris / 生物工程学报

According to the amino acid sequence of des-pGlu1-Brazzein, 4 pairs of oligonucleotide with cosmic site were synthesized by using yeasty biased codons. After linkage and PCR, the 179 bp code area of des-pGlu1-Brazzein was obtained and inserted into pPIC9K, which resulted in the recombinant expression vector pPIC9K-Bra. By digestion with Sal I, the lined pPIC9K-Bra was transformed into Pichia pastoris GS115 by electric shock. The results of expression indicted that the secreted target protein accounted for 51.6% of total protein in the supernatant and showed biological activity after purification.

Optimizing conditions for the expression of human beta defensin 3 and des-pGlu1-Brazzein in Escherichia coli and analysis of their activity / 生物工程学报

The inductive conditions for the flask-shaking of E.coli BL21-pET-hBD3-Bra had been optimized, at the same time, the expressed protein had been purified and analyzed. The effect of three factors which were IPTG concentration, induction time and temperature on growth of strain and on the yield of hBD3-Bra was analyzed in detail. The result indicated that the concentration of IPTG had little effect on the growth and the expression of target protein between 0.2-1 mmol/L, Biomass would be improved as time passed, but the target protein didn't increase obviously as the same time, temperature was the most important factor, the expressed level of hBD3-Bra, as high as about 35% of total cell protein, could be gained when strain was induced by IPTG under 30 degrees C. Further analysis showed the best temperature for growth was 30 degrees C-32 degrees C and for expression protein was 30 degrees C.The purified hBD3-Bra has a weak antimicrobial activity, but is 200 times sweeter than that of sucrose. After digested by thrombin and purified by affinity column, the natural des-pGlul-Brazzein also has 600-time sweetness of sucrose, and the recombinant hBD3 has a high antimicrobial activity again E. coli and S. aureus.

Speech development in monosyllables of Chinese 3-years old children / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate speech development in monosyllables of Chinese 3-year old children, to explore the difficulties of these words in these children, and to provide a reference for rehabilitation of deaf and dumb children.@*METHOD@#Speech material consists of 245 monosyllables in high and flat tone. 71 children between the ages of 2 year and 9 months to 3 year and 3 months (average 3 years) were involved in this investigation by oral imitation test. Proportions correct of every word were statistically analyzed.@*RESULT@#The proportions correct of these words ranged from 0.26 to 1.00 (median= 0.92). There are significant differences among proportions correct of those words (P <0.01).@*CONCLUSION@#It should be considered that monosyllables are different in difficulty to Chinese 3 years old children by oral imitation test when selecting materials and criteria of speech test for rehabilitation of deaf and dumb children.

A Survey of the Speech Development of Chinese Normal Children in Pronouncing Monosyllables / 听力学及言语疾病杂志

Objective To observe the speech development in monosyllables from Chinese children so as to establish references in the provision of rehabilitation to hearing impaired children.To study any causal relations of monosyllable production and the age factor,and the difference of these words.Methods 2 029 children of the ages from 3 year and 3 months to 6 year and 2 months were studied after they were divided into three age groups.Speech material consisted of 945 commonly used Chinese monosyllables.Each word was used for each test to 50 children or greater for each group.Results The percentage of correctly repeated words was within the rang of 0.76～1(median=0.97)in the young group,0.42～1(median=0.95)for the younger group and 0.40～1(median=0.94)for the youngest group.Statistically significant differences were found between each age(P